Russ — Provider: We are only as smart as everyone in the room.
DubbelHelix
A Patient's Laboratory

DubbelHelix (2011–2015) — cancer patient community portal and biomarker company. Stanford-licensed TGS diagnostic. Predecessor to Syncofy LLC.

The Science

BiomarkerTwo Gene Score (TGS): LMO2 + CD137/4-1BB
Disease targetDiffuse Large B-Cell Lymphoma (DLBCL)
Methodrt-PCR on FFPE tissue (no fresh frozen required)
Stanford licenseS10-292 (STAN-778), filed Oct 5, 2011
PatentUS 7,711,492 — Methods for diagnosing lymphoma
SignificanceIdentifies high-risk DLBCL patients for clinical trial enrollment
7-Market NHL Incidence Model — CombImmune Commercial Analysis
NHL incidence projections across 7 major markets 2002–2019

~7,000 addressable DLBCL cases/year in US alone. 7-market total projected at 140K by 2019. Underpinned pharma outreach to Pfizer, Sanofi, Rinat, Immungene.

The TGS assay ran on standard clinical pathology infrastructure — FFPE blocks already collected for diagnosis, no additional biopsy. The two-gene signature (LMO2 high + CD137 low = poor prognosis) stratified DLBCL patients into risk tiers actionable for trial enrollment and treatment selection.

Timeline

11
2011
DubbelHelix consulting inc. founded

Cancer patient community portal launched at dubbelhelix.com — forums for Brain Tumor, Breast Cancer, Leukemia, and Non-Hodgkins Lymphoma patients, care givers, and advocates. Tagline: "A Patient's Laboratory."

11
2011
Stanford TGS license S10-292 filed

Exclusive license application for the Two Gene Score (TGS) biomarker — LMO2 + CD137/4-1BB measured by rt-PCR in FFPE tissue — for DLBCL prognosis. Patent 7,711,492.

12
2012
ASH 2011 data presented

TGS validation data presented at ASH. NHL market analysis: 7-market model, ~7,000 cases/year addressable for diagnostic test. Pharma outreach to Pfizer, Sanofi, Rinat, Immungene.

15
2014–15
CombImmune Inc. — StartX Med Class 1

B2B/pharma-facing successor entity, accepted into the first StartX Med class (Stanford's healthcare accelerator). Founding team: Holbrook Kohrt MD PhD (Stanford immunologist, NK cell/ADCC pioneer), Russell Pachynski MD (Stanford/WashU hematology-oncology), and Jason Yonehiro (biotech entrepreneur). Combined Stanford licenses S10-292 (TGS) + S04-183. NCI CDA executed.

26
2026
Syncofy LLC — carrying the lineage forward

KOL intelligence infrastructure for pharma commercial teams. The data science approach from TGS — systematic measurement replacing expert opinion — applied to physician influence at scale.

CombImmune Founding Team

StartX Med — Class 1 · Stanford University
Holbrook Kohrt, MD PhD
Stanford University · Division of Oncology
Co-Founder · Scientific Lead
In memoriam · 1977–2016

Oncologist-immunologist and Stanford Assistant Professor, trained under Ron Levy in the Levy Lab on Lymphocytes and Lymphoma. Launched his independent lab in 2012. Died February 2016, age 38, from complications of severe hemophilia.

Kohrt established the mechanistic rationale for combining NK checkpoint release (anti-KIR) with tumor-targeting antibodies — a concept now embedded in multiple active clinical programs. His dual PhD in biostatistics and tumor immunology made him equally rigorous in trial design and bench science. At the time of his death, five novel patented immuno-oncology approaches were in active development.

Key Publications
Blood, 2011CD137 stimulation enhances the antilymphoma activity of anti-CD20 antibodies
Blood, 2014Anti-KIR antibody enhancement of anti-lymphoma activity of natural killer cells as monotherapy and in combination with anti-CD20 antibodies
JCI, 2014Targeting CD137 enhances the efficacy of cetuximab
Review, widely citedEnhancement of antibody-dependent cell mediated cytotoxicity: a new era in cancer treatment

Tributes: The Lancet · Clinical Cancer Research (AACR) · Blood/ASH Clinical News · PMC5084977

Russell Pachynski, MD
Washington University in St. Louis · Siteman Cancer Center · GU Oncology Research Director
Co-Founder · Immunology Lead
Pixie Bio · Active

Immunologist and Associate Professor of Medicine at WashU, where he serves as GU Oncology Research Director at the Siteman Cancer Center. Joined WashU faculty in 2014. ACS Research Scholar and Harrington Discovery Institute Scholar.

His core discovery: chemerin — an endogenous chemoattractant — is systematically down-regulated across tumor types. Restoring chemerin drives pDC, CD8+ T cell, and NK cell infiltration into the tumor microenvironment, suppressing tumor growth through immune surveillance and trafficking. This mechanism underpins both the original CombImmune thesis and his current work at Pixie BioSciences.

Pixie BioSciences — WashU OTM spinout built on Pachynski chemerin IP. Developing a tumor-targeted, prostate antigen-directed chemerin therapeutic. Extended IP lineage from CombImmune.
Key Data — Chemerin recruits tumor-infiltrating immune effectors
Chemerin effect on tumor infiltrating leukocytes — pDC, cDC, CD4+, CD8+, NK cells

Chemerin drives pDC, CD8+ T cell, and NK cell infiltration into the tumor microenvironment vs. control (* p<0.05). Source: Pachynski lab / CombImmune.

Key Publications
JEM, 2012The chemoattractant chemerin suppresses melanoma by recruiting natural killer cell antitumor defenses — landmark paper establishing chemerin as tumor suppressor
Front. Immunol., 2019Chemerin suppresses breast cancer growth by recruiting immune effector cells
bioRxiv, 2019Chemerin increases T-cell mediated cytotoxicity via modulation of a novel CMKLR1/PTEN/PD-L1 axis
JCO, 2026 (ASCO)Real-world genomic analysis of chemerin in prostate cancer and correlation with markers of immune infiltration and activation
Jason Yonehiro
Syncofy LLC
Co-Founder · Operations & Business

Biotech entrepreneur. Holder of Stanford TGS license S10-292. Led operations, business development, and NCI CDA execution for DubbelHelix and CombImmune. Built the pharma outreach pipeline (Pfizer, Sanofi, Rinat, Immungene) and ASH market analysis. DubbelHelix → CombImmune → Syncofy LLC.

The Patient Portal (2011–2012)

DubbelHelix.com ran cancer-specific forums where patients, care givers, and advocates could ask questions anonymously and connect with others navigating the same diagnosis. The platform had separate boards for Brain Tumor, Breast Cancer, Leukemia, and Non-Hodgkins Lymphoma, with a real-time health news feed aggregating ASCO, ASH, and American Cancer Society releases.

Forum topics
Brain Tumor · Breast Cancer · Leukemia · NHL
User types
Patient · Care Giver · Advocate · Provider
Built
2011 — PHP/MySQL, hosted at Arvixe
Original Marketing Brochure — 2012
DubbelHelix original brochure — We Deliver Results
"We Deliver Results"

The original DubbelHelix patient portal brochure distributed in 2012. Tagline: "Whether they be care takers, cancer advocates or real cancer survivors, this web portal is the perfect destination for anonymous discussion about questions you may have about cancer."

dubbelhelix.com · 2012

DubbelHelix Community — Follow & Connect

Active since 2011 · Facebook archive (2011–2015)

Site Archive — 2011–2012

Screenshots from the original DubbelHelix.com patient portal, captured during active operation. Forums, community boards, and the homepage as they appeared to patients and caregivers.

Homepage — 2011

Homepage — 2011

Homepage variant — 2011

Homepage variant — 2011

Forum — 2012

Forum — 2012

Community page — 2012

Community page — 2012

Patient discussions — 2012

Patient discussions — 2012

Connect page — 2012

Connect page — 2012

DubbelHelix → CombImmune → Syncofy

The core insight from DubbelHelix — that systematic measurement of biological signal outperforms clinical intuition for stratifying patients — translates directly to what Syncofy does for KOL identification. Replace "which DLBCL patients are high-risk?" with "which physicians are actually driving clinical practice change?" and the methodology is the same: score against raw data, not reputation networks.